Optional Downstream Analysis Tools

Generate Protein Fasta

usage: pvacseq generate_protein_fasta [-h] [-d DOWNSTREAM_SEQUENCE_LENGTH]
                                      input_file peptide_sequence_length
                                      output_file
Required Arguments
input_file A VEP-annotated single-sample VCF containing transcript, Wildtype protein sequence, and Downstream protein sequence information
peptide_sequence_length
 Length of the peptide sequence to use when creating the FASTA.
output_file The output fasta file
Optional Arguments
-d="1000", --downstream-sequence-length="1000"
 Cap to limit the downstream sequence length for frameshifts when creating the fasta file. Use ‘full’ to include the full downstream sequence. Default: 1000